Expression of Cyclin D1, Cyclin E, p21Cip1 and p27Kip1 in Chemically Induced Rat Mammary Tumor Treated with Tamoxifen and Transforming Growth Factor-1
Korean Journal of Pathology
;
: 151-157, 2001.
Article
in Korean
| WPRIM
| ID: wpr-183405
ABSTRACT
BACKGROUND:
Tamoxifen (TAM) inhibits the action of estrogen by binding to estrogen receptors, and also has non-estrogen receptor mediated cytostatic activities. Transforming growth factor-1 (TGF-1) inhibits the proliferation of many other cell types, such as epithelial, hematopoietic and endothelial cells.METHODS:
We investigated the effects of tamoxifen on the growth of 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary tumors and the expression of cyclin D1, cyclin E, p21Cip1, and p27Kip1 by performing immunohistochemistry and Western blot analysis, and studied whether TGF-1 injection amplified the effects of TAM. When tumor size reached between 10-15 mm in the largest dimension, the rats were divided into 3 groups DMBA-control group (n=12), DMBA-TAM group (n=14) and DMBA-TAM plus TGF-1 group (n=5).RESULTS:
The consecutive administration of TAM markedly decreased the tumor development compared with the DMBA-control group. The DMBA-TAM and DMBA-TAM plus TGF-1 groups showed decreased expression of bromodexoyuridine, cyclin D1, cyclin E, and p21Cip1 when compared with those of the DMBA-control group. On the other hand, the labeling index of p27Kip1 was higher in the DMBA-TAM plus TGF-1 group than in the DMBA-control group.CONCLUSION:
TAM suppresses tumor development, which may be associated with down-expression of cyclin D1 and cyclin E, and overexpression of p27Kip1, and addition of TGF-1 does not influence tumor development treated by TAM.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Robenidine
/
Tamoxifen
/
Breast
/
Immunohistochemistry
/
Receptors, Estrogen
/
Cell Cycle
/
Blotting, Western
/
Transforming Growth Factor beta
/
Cyclins
/
Cyclin D1
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS