High Glucose and Advanced Glycosylation Endproducts(AGE) Modulate the P-cadherin Expression in Glomerular Epithelial Cells(GEpC)
Journal of the Korean Society of Pediatric Nephrology
;
: 119-127, 2005.
Article
in Korean
| WPRIM
| ID: wpr-184966
ABSTRACT
PURPOSE:
Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the integrity of slit diaphragm(SD) proteins including nephrin, P-cadherin, and others. Diabetic proteinuric condition demonstrates defects in SD molecules as well as ultrastructural changes in podocytes. We examined the molecular basis for this alteration of SD molecules especially on P-cadherin as a candidate regulating the modulation of pathogenic changes in the barrier to protein filtration.METHODS:
To investigate whether high glucose and AGE induce changes in SD, we cultured rat GEpC under normal(5 mM) or high glucose(30 mM) and AGE- or BSA-added conditions and measured the change of P-cadherin expression by Western blotting and RT- PCR.RESULTS:
We found that administration of high glucose decreased the P-cadherin production significantly in the presence or absence of AGE by Western blotting. In RT-PCR high glucose with or without AGE also significantly decreased the expression of P-cadherin mRNA compared to those of controls. Such changes were not seen in the osmotic control.CONCLUSION:
We suggest that high glucose with or without AGE suppresses the production of P-cadherin at the transcriptional level and that these changes may explain the functional changes of SD in diabetic conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Glycosylation
/
RNA, Messenger
/
Cadherins
/
Blotting, Western
/
Polymerase Chain Reaction
/
Diabetic Nephropathies
/
Podocytes
/
Filtration
/
Glucose
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Society of Pediatric Nephrology
Year:
2005
Type:
Article
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