In vitro MRI and Characterization of Rat Mesenchymal Stem Cells Transduced with Ferritin as MR Reporter Gene
Journal of the Korean Society of Magnetic Resonance in Medicine
;
: 47-54, 2012.
Article
in English
| WPRIM
| ID: wpr-185403
ABSTRACT
PURPOSE:
This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate in vitro MRI detectability of ferritin-transduced RMSCs. MATERIALS ANDMETHODS:
The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. In vitro magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs.RESULTS:
The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower T2 relaxation time than non-transduced RMSCs.CONCLUSION:
Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apoferritins
/
Relaxation
/
Track and Field
/
Magnetic Resonance Imaging
/
Fluorescent Antibody Technique
/
Genes, Reporter
/
Ferritins
/
Ferrocyanides
/
Mesenchymal Stem Cells
/
Flow Cytometry
Limits:
Animals
/
Humans
Language:
English
Journal:
Journal of the Korean Society of Magnetic Resonance in Medicine
Year:
2012
Type:
Article
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