Post-mortem re-cloning of a transgenic red fluorescent protein dog
Journal of Veterinary Science
;
: 405-407, 2011.
Article
in English
| WPRIM
| ID: wpr-186139
ABSTRACT
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Trachea
/
Animals, Genetically Modified
/
Gene Expression Regulation
/
Cloning, Organism
/
Gastrointestinal Tract
/
Dogs
/
Nuclear Transfer Techniques
/
Kidney
/
Liver
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2011
Type:
Article
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