Co-activation of Gi and Gq proteins exerts synergistic effect on human platelet aggregation through activation of phospholipase C and Ca2+ signalling pathways
Experimental & Molecular Medicine
;
: 42-46, 1999.
Article
in English
| WPRIM
| ID: wpr-186198
ABSTRACT
Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet aggregation. We show that 5-HT had no or little effect on aggregation but it did potentiate the aggregation response of epinephrine. The synergistic interaction of 5-HT (1-5 microM) and epinephrine (0.5-2 microM) was inhibited by alpha2-adrenoceptor blocker (yohimbine; IC50= 0.4 microM), calcium channel blockers (verapamil and diltiazem with IC50 of 10 and 48 mM, respectively), PLC inhibitor (U73122; IC50=6 microM) and nitric oxide (NO) donor, SNAP (IC50=1.6 microM)). The data suggest that synergistic effects of platelet agonists are receptor-mediated and occur through multiple signalling pathways including the activation PLC/Ca2+ signalling cascades.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Type C Phospholipases
/
Calcium Channel Blockers
/
Signal Transduction
/
Epinephrine
/
Serotonin
/
Platelet Aggregation
/
Blotting, Western
/
GTP-Binding Proteins
/
GTP-Binding Protein alpha Subunits, Gi-Go
/
Calcium Signaling
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1999
Type:
Article
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