Involvement of Protein Kinase C-delta in Vascular Permeability in Acute Lung Injury
Immune Network
;
: 206-211, 2015.
Article
in English
| WPRIM
| ID: wpr-186449
ABSTRACT
Pulmonary edema is a major cause of mortality due to acute lung injury (ALI). The involvement of protein kinase C-delta (PKC-delta) in ALI has been a controversial topic. Here we investigated PKC-delta function in ALI using PKC-delta knockout (KO) mice and PKC inhibitors. Our results indicated that although the ability to produce proinflammatory mediators in response to LPS injury in PKC-delta KO mice was similar to that of control mice, they showed enhanced recruitment of neutrophils to the lung and more severe pulmonary edema. PKC-delta inhibition promoted barrier dysfunction in an endothelial cell layer in vitro, and administration of a PKC-delta-specific inhibitor significantly increased steady state vascular permeability. A neutrophil transmigration assay indicated that the PKC-delta inhibition increased neutrophil transmigration through an endothelial monolayer. This suggests that PKC-delta inhibition induces structural changes in endothelial cells, allowing extravasation of proteins and neutrophils.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Kinases
/
Pulmonary Edema
/
Capillary Permeability
/
Mortality
/
Endothelial Cells
/
Protein Kinase C-delta
/
Acute Lung Injury
/
Lung
/
Neutrophils
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2015
Type:
Article
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