Changes in Expression of BCL-2, BAX and ICE mRNA After Middle Cerebral Artery Occlusion in Rat
Journal of the Korean Neurological Association
;
: 568-573, 1999.
Article
in Korean
| WPRIM
| ID: wpr-18647
ABSTRACT
BACKGROUND:
In order to delineate the involvement of apoptotic death in post-ischemic cerebral infarction, the temporal expressions of some apoptosis-regulating genes (BCL-2, BAX and ICE) were studied in the parietofrontal cortex of a rat following focal cerebral ischemia using a Northern blot analysis and RT-PCR.METHODS:
Animals were decapitated at 0.5, 1, 2, 4, 8 and 24 hours following a middle cerebral artery occlusion (MCAO).RESULTS:
With the use of Northern blotting, the expression of proto-oncogene BCL-2, the apoptosis-repressor gene, increased up to 4 hours after a MCAO and then recovered to the control level. In a RT-PCR analysis, the expression of BCL-2 increased up to 8 hours after the MCAO and then recovered to the control level at 24 hours. The expression of BAX, the apoptosis-effec-tor gene, was not altered by a MCAO in a Northern blot analysis. The expression of ICE, an cysteine protease gene, markedly decreased at 1 and 2 hours after the MCAO and then recovered to the control level.CONCLUSIONS:
These results show that ischemic insults by a MCAO alter the expression of the apoptosis-regulating genes and support the notion that the apoptosis may have occurred in the post-ischemic cerebral infarction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proto-Oncogenes
/
RNA, Messenger
/
Cerebral Infarction
/
Brain Ischemia
/
Blotting, Northern
/
Apoptosis
/
Middle Cerebral Artery
/
Infarction, Middle Cerebral Artery
/
Cysteine Proteases
/
Ice
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
1999
Type:
Article
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