Distribution of 5-HT3, 5-HT4, and 5-HT7 Receptors Along the Human Colon
Journal of Neurogastroenterology and Motility
;
: 361-369, 2015.
Article
in English
| WPRIM
| ID: wpr-186686
ABSTRACT
BACKGROUND/AIMS:
Several disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT3 and 5-HT4 receptors are clinically relevant. The aim was to examine the distribution of 5-HT3, 5-HT4, and 5-HT7 receptors in the normal human colon and how this is associated with receptor interacting chaperone 3, G protein coupled receptor kinases, and protein LIN-7 homologs to extend previous observations limited to the sigmoid colon or the upper intestine.METHODS:
Samples from ascending, transverse, descending, and sigmoid human colon were dissected into 3 separate layers (mucosa, longitudinal, and circular muscles) and ileum samples were dissected into mucosa and muscle layers (n = 20). Complementary DNA was synthesized by reverse transcription from extracted RNA and expression was determined by quantitative or end point polymerase chain reaction.RESULTS:
The 5-HT3 receptor subunits were found in all tissues throughout the colon and ileum. The A subunit was detected in all samples and the C subunit was expressed at similar levels while the B subunit was expressed at lower levels and less frequently. The 5-HT3 receptor E subunit was mainly found in the mucosa layers. All splice variants of the 5-HT4 and 5-HT7 receptors were expressed throughout the colon although the 5-HT4 receptor d, g, and i variants were expressed less often.CONCLUSIONS:
The major differences in 5-HT receptor distribution within the human colon are in relation to the mucosa and muscular tissue layers where the 5-HT3 receptor E subunit is predominantly found in the mucosal layer which may be of therapeutic relevance.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Colon, Sigmoid
/
RNA
/
Serotonin
/
Polymerase Chain Reaction
/
Receptors, Serotonin
/
Colon
/
DNA, Complementary
/
Gastrointestinal Tract
/
Receptors, Serotonin, 5-HT4
/
Receptors, Serotonin, 5-HT3
Limits:
Humans
Language:
English
Journal:
Journal of Neurogastroenterology and Motility
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS