The effect of fentanyl and midazolam on in vitro fertilization and early development of mouse embryo / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 955-960, 2000.
Article
in Korean
| WPRIM
| ID: wpr-187011
ABSTRACT
OBJECTIVE:
To assess the effect of fentanyl and midazolam on in vitro fertilization rate and early embryo development in a mouse IVF model.METHODS:
Mouse oocytes were exposed in vitro to fentanyl at a concentration of 0(control), 50, 250, 500, 1000, 5000 pg/ml, and midazolam, 0(control), 2.5, 12.5, 25, 50, 250 ng/ml for 30 minutes, washed and inseminated. Thereafter fertilization was assessed. And subsequent in vitro development to the blastocyst stage was monitored daily.RESULTS:
Where fertilization occurred, subsequent embryo cleavage and development up to the blastocyst stage was affected significantly by the presence of fentanyl and midazolam solution in the medium(i.e., 14% to 31%, 10% to 35%), in comparison with control group( 60%, 62%).CONCLUSION:
It can be concluded from these experiments that even a brief exposure of cumulus enclosed oocytes to a low concentration of fentanyl, midazolam is deleterious to subsequent cleavage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Blastocyst
/
Midazolam
/
Fertilization in Vitro
/
Fentanyl
/
Embryonic Development
/
Embryonic Structures
/
Fertilization
/
Anesthetics
Limits:
Animals
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2000
Type:
Article
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