Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury
The Korean Journal of Internal Medicine
;
: 1053-1061, 2017.
Article
in English
| WPRIM
| ID: wpr-187141
ABSTRACT
BACKGROUND/AIMS:
Recent findings have demonstrated the occurrence of neutrophil transendothelial migration in the reverse direction (reverse TEM) and that endothelial junctional adhesion molecule C (JAM-C) is a negative regulator of reverse TEM. In this study, we tested the effects of a JAM-C blocking antibody on the resolution of kidney injuries and inflammation in a mouse model of cisplatin-induced acute kidney injury (AKI).METHODS:
Cisplatin was administered via intraperitoneal injection. A JAM-C blocking antibody or a control immunoglobulin G was administered intraperitoneal at 1.5 mg/kg, with the injection being delayed until day 4 following cisplatin administration to restrict the effect of antibodies on recovery.RESULTS:
After cisplatin injection, serum creatinine and histologic injuries peaked on day 4. Treatment with a JAM-C blocking antibody on days 4 and 5 promoted the functional and histologic recovery of cisplatin-induced AKI on days 5 and 6. Facilitating recovery with a JAM-C blocking antibody correlated with significantly increased circulating intercellular adhesion molecule 1+ Tamm-Horsfall protein+ neutrophils and significantly decreased renal neutrophil infiltration, indicating that facilitating reverse the TEM of neutrophils from the kidney to the peripheral circulation partially mediated the resolution of inflammation and recovery.CONCLUSIONS:
These results demonstrated that reverse TEM is involved in the resolution of neutrophilic inflammation in cisplatin-induced AKI and that JAM-C is an important regulator of this process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin G
/
Cisplatin
/
Creatinine
/
Neutrophil Infiltration
/
Acute Kidney Injury
/
Transendothelial and Transepithelial Migration
/
Junctional Adhesion Molecules
/
Junctional Adhesion Molecule C
/
Inflammation
/
Injections, Intraperitoneal
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Internal Medicine
Year:
2017
Type:
Article
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