Serologic Variability of the A(var) (784G>A) and Its Property of Different Expression Depending on Co-inherited ABO Allele / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 61-70, 2006.
Article
in Korean
| WPRIM
| ID: wpr-187477
ABSTRACT
BACKGROUND:
A allele, A(var), characterized by a 784G>A polymorphism (Asp262Asn) has been identified only in Korean A(weak)B donors. This study evaluated the serological and genetic characteristics of thirteen samples with newly identified A(var) allele.METHODS:
This study examined 10 samples with the A(var) allele including 4 members from a family, who were randomly obtained from blood donors recruited at Gwangju-Chonnam Red Cross Blood Center, and patients at the Chonnam National University Hospital. Routine ABO serologic tests, ABO genotyping using an allele specific polymerase chain reaction (AS-PCR), and the sequencing of exon 6 and 7 of ABO gene were performed on all samples. In addition, sequencing of exon 1~5 of the ABO gene was carried out on two randomly selected samples.RESULTS:
The A(var) allele was identified in nine A(weak)B and one O (II-1 of the family study) sample. Eight of these nine individuals showed 1+ agglutination with the monoclonal anti-A reagents on forward typing but one sample showed no agglutination. Weak anti-A was detected in all sera. From the family study, the A(var) allele, which was transmitted from the propositus through her descendant (II-1, II-3 and III-1), produced either the weak A phenotype when inherited with a B allele or the O phenotype when inherited with an O allele.CONCLUSION:
A(var) erythrocytes showed different agglutination patterns to anti-A. Different expressions (possible allelic enhancement) were observed depending on the co-inherited ABO alleles from samples with the A(var) allele.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Red Cross
/
Tissue Donors
/
Blood Donors
/
Serologic Tests
/
Polymerase Chain Reaction
/
Exons
/
Agglutination
/
Alleles
/
Erythrocytes
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2006
Type:
Article
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