The Correlation between the S/CO Ratio of Anti-HCV ELISA, and the Results of the RIBA and RNA Test / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 54-60, 2006.
Article
in Korean
| WPRIM
| ID: wpr-187478
ABSTRACT
BACKGROUND:
Recombinant immunoblot assay (RIBA) or RNA test is considered to be a supplemental test for confirming a HCV infection. A correlation has been reported between the signal-to-cutoff (S/CO) ratios of a third generation HCV enzyme-linked immunosorbent assay (ELISA) and a confirmed HCV infection. This study examined the results of an evaluation of domestic anti-HCV EIA and immunoblot kit (RIBA) in Korean donors.METHODS:
A total of 375,576 donor samples were tested for anti-HCV using the LG third generation HCV ELISA (LG HCD 3.0 TMB, LGphD, Korea) and HCV RNA by NAT (Biomerieux/Roche RT-PCR, 24 pool). The anti-HCV repeat reactive samples were further tested by third generation RIBA (LG HCD Confirm, LGphD, Korea). A positive result by either the nucleic acid amplification test (NAT) or RIBA was interpreted as a confirmed HCV infection.RESULTS:
There were 506 out of the 375,576 donor samples (0.13%) that were anti-HCV repeat reactive (RR) by routine screening ELISA. The confirmed HCV prevalence in the donors was 0.01% (RIBA 42/375,570, RNA 36/375,570). 443 samples from the 506 repeat reactive samples in ELISA (87.6%) showed a S/CO ratio 3.6 (mean 4.40+/-0.80), compared with the negative group (mean 1.54+/-0.64).CONCLUSION:
There was a good correlation between a high S/CO ratios and a confirmed HCV infection. In addition, samples showing a low S/CO ratio with an ID (Indeterminate) or negative RIBA result suggest a high probability of nonspecific reactivity in ELISA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
RNA
/
Enzyme-Linked Immunosorbent Assay
/
Mass Screening
/
Prevalence
/
Nucleic Acid Amplification Techniques
Type of study:
Prevalence study
/
Screening study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2006
Type:
Article
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