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Comparison of Batch Assay and Random Assay Using Automatic Dispenser in Radioimmunoassay / 핵의학분자영상
Nuclear Medicine and Molecular Imaging ; : 323-329, 2009.
Article in Korean | WPRIM | ID: wpr-187528
ABSTRACT

PURPOSE:

Radioimmunoassay (RIA) was usually performed by the batch assay. To improve the efficiency of RIA without increase of the cost and time, random assay could be a choice. We investigated the possibility of the random assay using automatic dispenser by assessing the agreement between batch assay and random assay. MATERIALS AND

METHODS:

The experiments were performed with four items; Triiodothyronine (T3), free thyroxine (fT4), Prostate specific antigen (PSA), Carcinoembryonic antigen (CEA). In each item, the sera of twenty patients, the standard, and the control samples were used. The measurements were done 4 times with 3 hour time intervals by random assay and batch assay. The coefficient of variation (CV) of the standard samples and patients' data in T3, fT4, PSA, and CEA were assessed. ICC (Intraclass correlation coefficient) and coefficient of correlation were measured to assessing the agreement between two methods.

RESULTS:

The CVs (%) of T3, fT4, PSA, and CEA measured by batch assay were 3.2+/-1.7%, 3.9+/-2.1%, 7.1+/-6.2%, 11.2+/-7.2%. The CVs by random assay were 2.1+/-1.7%, 4.8+/-3.1%, 3.6+/-4.8%, and 7.4+/-6.2%. The ICC between the batch assay and random assay were 0.9968 (T3), 0.9973 (fT4), 0.9996 (PSA), and 0.9901 (CEA). The coefficient of correlation between the batch assay and random assay were 0.9924(T3), 0.9974 (fT4), 0.9994 (PSA), and 0.9989 (CEA) (p<0.05).

CONCLUSION:

The results of random assay showed strong agreement with the batch assay in a day. These results suggest that random assay using automatic dispenser could be used in radioimmunoassay.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Thyroxine / Triiodothyronine / Radioimmunoassay / Carcinoembryonic Antigen / Prostate-Specific Antigen Type of study: Controlled clinical trial Limits: Humans Language: Korean Journal: Nuclear Medicine and Molecular Imaging Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Thyroxine / Triiodothyronine / Radioimmunoassay / Carcinoembryonic Antigen / Prostate-Specific Antigen Type of study: Controlled clinical trial Limits: Humans Language: Korean Journal: Nuclear Medicine and Molecular Imaging Year: 2009 Type: Article