Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice
Experimental & Molecular Medicine
; : 197-204, 2011.
Article
in En
| WPRIM
| ID: wpr-187633
Responsible library:
WPRO
ABSTRACT
To determine the impact of IL-23 knockdown by RNA interference on the development and severity of ovalbumin (OVA)-induced asthmatic inflammation, and the potential mechanisms in mice, the IL-23-specific RNAi-expressing pSRZsi-IL-23p19 plasmid was constructed and inhaled into OVA-sensitized mice before each challenge, as compared with that of control mice treated with alum or budesonide. Inhalation of the pSRZsi-IL-23p19, significantly reduced the levels of OVA-challenge induced IL-23 in the lung tissues by nearly 75%, determined by RT-PCR. In addition, knockdown of IL-23 expression dramatically reduced the numbers of eosinophils and neutrophils in BALF and mitigated inflammation in the lungs of asthmatic mice. Furthermore, knockdown of IL-23 expression significantly decreased the levels of serum IgE, IL-23, IL-17, and IL-4, but not IFNgamma, and its anti-inflammatory effects were similar to or better than that of treatment with budesonide in asthmatic mice. Our data support the notion that IL-23 and associated Th17 responses contribute to the pathogenic process of bronchial asthma. Knockdown of IL-23 by RNAi effectively inhibits asthmatic inflammation, which is associated with mitigating the production of IL-17 and IL-4 in asthmatic mice.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Asthma
/
Enzyme-Linked Immunosorbent Assay
/
Bronchoalveolar Lavage Fluid
/
Ovalbumin
/
Reverse Transcriptase Polymerase Chain Reaction
/
RNA, Small Interfering
/
RNA Interference
/
Eosinophils
/
Interleukin-23
Limits:
Animals
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2011
Type:
Article