Hydroxydibenzoylmethane induces apoptosis through repressing ornithine decarboxylase in human promyelocytic leukemia HL-60 cells
Experimental & Molecular Medicine
;
: 189-196, 2011.
Article
in English
| WPRIM
| ID: wpr-187634
ABSTRACT
Ornithine decarboxylase (ODC) is the rate-limiting enzyme in polyamine biosynthesis and a target for chemoprevention. Hydroxydibenzoylmethane (HDB), a derivative of dibenzoylmethane of licorice, is a promising chemopreventive agent. In this paper, we investigated whether HDB would inhibit the ODC pathway to enhance apoptosis in human promyelocytic leukemia HL-60 cells. We found ODC enzyme activity was reduced during HDB treatment. Overexpression of ODC in HL-60 parental cells could reduce HDB-induced apoptosis, which leads to loss of mitochondrial membrane potential (Deltapsim), through lessening intracellular ROS. Furthermore, ODC overexpression protected cytochrome c release and the activation of caspase-3 following HDB treatment. The results demonstrated HDB-induced apoptosis was through a mechanism of down-regulation of ODC and occurred along a ROS-dependent mitochondria-mediated pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ornithine Decarboxylase
/
Immunoblotting
/
Leukemia, Myeloid
/
Down-Regulation
/
Gene Expression
/
Reactive Oxygen Species
/
Apoptosis
/
HL-60 Cells
/
Chemoprevention
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2011
Type:
Article
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