Effects of Mechanical Stimulation and Scaffolds for Nucleus Pulposus Replacement of Intervertebral Disc / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 45-52, 2009.
Article
in Korean
| WPRIM
| ID: wpr-187826
ABSTRACT
PURPOSE:
In this study, we investigated the potential of injectable hydrogel scaffolds for the regeneration of nucleus pulposus. MATERIALS ANDMETHODS:
We prepared injectable hydrogels [Chitosan-Pluronic (CP), CP/Osteogenic Protein-1 (CP/OP-1), CP/Gly-Arg-Gly-Asp-Ser (CP/GRGDS), CP/GRGDS/OP-1] for this study. One of the four potential materials was selected through the cell viability tests. For each material, primary cultured nucleus pulposus (NP) cells from New Zealand rabbits were seeded onto each material. For the investigation of the effects of mechanical stimulation, the commercially available bioreactor was used. 0.2 MPa of intermittent hydrostatic pressure was imposed for 3 days after 7th day of seeding with the pattern of 2 min and 15 min for stimulating and resting, respectively. The specimens were harvested at 1, 10, 14 day after seeding for analyses.RESULTS:
The MTT assay for 5 days revealed that CP/OP-1 group showed significant increase. The other two groups (CP/GRGDS and CP/GRGDS/OP-1) showed that the proliferation rate increased until 3 days after culture, while it decreased on day 5. The mechanical stimuli induced higher amounts of DNA measured in CP/OP- 1 on day 5 after culture. However, no significant difference was observed between two groups.CONCLUSION:
We came to the conclusions that the biochemical environment as well as mechanical stimulation may play an important role in regenerating nucleus pulposus matrix, especially in CP/OP-1 in this study. However, further study are recommended in relation to mechanical effects as well as biochemical conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Porphyrins
/
Regeneration
/
Seeds
/
DNA
/
Cell Survival
/
Bioreactors
/
Hydrogels
/
Hydrostatic Pressure
/
Intervertebral Disc
Limits:
Animals
Language:
Korean
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2009
Type:
Article
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