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Evaluation of Positive Rate of Aerobic BacT/Alert Blood Culture Bottles by Antibiotic Usage and Inoculated Blood Volume / 대한임상병리학회지
Korean Journal of Clinical Pathology ; : 343-349, 2001.
Article in Korean | WPRIM | ID: wpr-18789
ABSTRACT

BACKGROUND:

Inoculated blood volume and residual antibiotic effects are the key variables that influence successful blood cultures especially in patients receiving antibiotics. In our hospital we have inoculated BacT/Alert blood culture bottles with 5 mL of blood, but recent reports about the BacT/Alert system recommend an inoculation of up to 10 mL of blood.

METHODS:

We studied about 931 paired aerobic blood culture sets and each set was inoculated with 3 mL (3 mL bottle) and 7 mL (7 mL bottle) of blood. The positive rates, the yield and speed of detecting microorganisms in the culture bottles were compared to the specimen information of antibiotic usage. We also analyzed the antibiotic effect on each set of blood cultures by inoculation with standard microorganisms (E. coli ATCC 25922 and S. aureus ATCC 25923) in the used bottle sets.

RESULTS:

Positive cultures were obtained from 98 sets (10.5%) and 161 organisms grew. Of 931 blood culture bottle sets, 744 (79.9%) were obtained from patients on antibiotic therapy. The positive rate of blood culture bottles from patients receiving antibiotic therapy was significantly lower than those from patients who were not receiving antibiotic therapy (P0.05). When culture bottles with no growth (from patients receiving antibiotic therapy) were used, no differences were found in detection time and positive rates between 3 mL and 7 mL blood culture bottles that were artificially seeded with standard E. coli or S. aureus strains.

CONCLUSIONS:

Although antibiotic usage rate before using the blood cultures was high in our hospital, the 7 mL bottle showed a better yield and speed of detecting microorganisms than did the 3 mL bottle irrespective of antibiotic usage before using the blood cultures. This data suggests that blood volume elevation (from 5 mL up to 10 mL) can be recommended for BacT/Alert Blood culture systems.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood Volume / Anti-Bacterial Agents Limits: Humans Language: Korean Journal: Korean Journal of Clinical Pathology Year: 2001 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood Volume / Anti-Bacterial Agents Limits: Humans Language: Korean Journal: Korean Journal of Clinical Pathology Year: 2001 Type: Article