Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea
The Korean Journal of Parasitology
;
: 85-89, 2004.
Article
in English
| WPRIM
| ID: wpr-188031
ABSTRACT
Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI) ; but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Zoonoses
/
Polymerase Chain Reaction
/
Cryptosporidium parvum
/
Cryptosporidiosis
/
Oocysts
/
Diarrhea
/
Feces
/
Genotype
/
Korea
/
Mice, Inbred BALB C
Limits:
Animals
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2004
Type:
Article
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