Percentage of motile spermatozoa at 22 hours after swim-up procedure: An indicator for intracytoplasmic sperm injection? / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 157-163, 2016.
Article
in English
| WPRIM
| ID: wpr-188153
ABSTRACT
OBJECTIVE:
The decision to use in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), or split insemination (IVF-ICSI) in the first cycle is based on the number of motile sperm. Hence, total fertilization failure (TFF) often occurs during IVF cycles, despite normozoospermia. To investigate whether the cumulative motile swim-up spermatozoa percentage at 22 hours post-insemination (MSPPI) is an indicator for ICSI, we analyzed TFF, fertilization, blastocyst development, chemical pregnancy, clinical pregnancy, and live birth rates.METHODS:
This prospective study was performed using data obtained from 260 IVF cycles. At 22 hours after insemination, the remaining swim-up spermatozoa were observed and divided into six groups according to MSPPI (<10%, 10% to <30%, 30% to <50%, 50% to <70%, 70% to <90%, and 90% to 100%).RESULTS:
Regardless of the ejaculated motile sperm concentration (0.6–280×106/mL motile spermatozoa), the incidence of TFF significantly increased when MSPPI was <10%, and the fertilization rate significantly decreased when MSPPI was <30%. We found that cumulative MSPPI correlated with the cumulative fertilization rate (Spearman correlation, 0.508, p<0.001). Regarding embryo development, we observed no significant differences in the rates of blastocyst development, chemical pregnancy, clinical pregnancy, or live birth among all groups.CONCLUSION:
Our findings suggest that MSPPI is a viable indicator for split IVF-ICSI and ICSI. Taken together, by employing the MSPPI test in advance before IVF, ICSI, or split IVF-ICSI cycles, unnecessary split IVF-ICSI and ICSI may be avoided.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatozoa
/
Blastocyst
/
Fertilization in Vitro
/
Incidence
/
Prospective Studies
/
Sperm Injections, Intracytoplasmic
/
Embryonic Development
/
Live Birth
/
Fertilization
/
Insemination
Type of study:
Incidence study
/
Observational study
/
Prognostic study
Limits:
Pregnancy
Language:
English
Journal:
Clinical and Experimental Reproductive Medicine
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS