Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis
The Korean Journal of Parasitology
;
: 105-108, 2008.
Article
in English
| WPRIM
| ID: wpr-188649
ABSTRACT
Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH strains exhibited typical PCR-RFLP patterns identical to the type I strains. DNA sequencing of tandem repeats in 5 microsatellite markers (B17, B18, TUB2, W35, and TgM-A) of the KI-1 also revealed patterns characteristic of the type I. These results provide strong genetic evidence that KI-1 is a type I lineage of T. gondii.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Toxoplasma
/
Polymorphism, Restriction Fragment Length
/
Molecular Sequence Data
/
Base Sequence
/
Cell Line
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Sequence Alignment
/
Microsatellite Repeats
/
Genotype
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2008
Type:
Article
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