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Occult HBV among Anti-HBc Alone: Mutation Analysis of an HBV Surface Gene and Pre-S Gene
Yonsei Medical Journal ; : 557-563, 2017.
Article in English | WPRIM | ID: wpr-188814
ABSTRACT

PURPOSE:

The aim of this study is to investigate the molecular characteristics of occult hepatitis B virus (HBV) infection in ‘anti-HBc alone’ subjects. MATERIALS AND

METHODS:

Twenty-four patients with ‘anti-HBc alone’ and 20 control patients diagnosed with HBV were analyzed regarding S and pre-S gene mutations. All specimens were analyzed for HBs Ag, anti-HBc, and anti-HBs. For specimens with an anti-HBc alone, quantitative analysis of HBV DNA, as well as sequencing and mutation analysis of S and pre-S genes, were performed.

RESULTS:

A total 24 were analyzed for the S gene, and 14 were analyzed for the pre-S gene through sequencing. A total of 20 control patients were analyzed for S and pre-S gene simultaneously. Nineteen point mutations of the major hydrophilic region were found in six of 24 patients. Among them, three mutations, S114T, P127S/T, M133T, were detected in common. Only one mutation was found in five subjects of the control group; this mutation was not found in the occult HBV infection group, however. Pre-S mutations were detected in 10 patients, and mutations of site aa58–aa100 were detected in 9 patients. A mutation on D114E was simultaneously detected. Although five mutations from the control group were found at the same location (aa58–aa100), no mutations of occult HBV infection were detected.

CONCLUSION:

The prevalence of occult HBV infection is not low among ‘anti-HBc alone’ subjects. Variable mutations in the S gene and pre-S gene were associated with the occurrence of occult HBV infection. Further larger scale studies are required to determine the significance of newly detected mutations.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / Hepatitis B virus / Prevalence / Point Mutation Type of study: Prevalence study Limits: Humans Language: English Journal: Yonsei Medical Journal Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / Hepatitis B virus / Prevalence / Point Mutation Type of study: Prevalence study Limits: Humans Language: English Journal: Yonsei Medical Journal Year: 2017 Type: Article