Derivation of Androgen-Independent LNCaP Sublines from Cell-Cell Interactions of LNCaP and Human Prostate Fibroblasts in vivo / 대한비뇨기과학회지
Korean Journal of Urology
;
: 1478-1486, 1999.
Article
in Korean
| WPRIM
| ID: wpr-18893
ABSTRACT
PURPOSE:
A cell-cell interaction in which in vivo inoculation of androgen-dependent, non-tumorigenic LNCaP and human bone fibroblast resulted in derivation of androgen-independent and metastatic LNCaP subline(C4-2) in castrated hosts. The purpose of this study is to evaluate if human prostate fibroblasts when grown together with LNCaP may promote androgen-independent growth and enhance metastatic potential. MATERIALS ANDMETHODS:
LNCaP cells and human prostate fibroblasts derived either from peripheral or transition zone co-inoculated in athymic mice for 8 weeks, and then mice were castrated. The chimeric tumors were maintained for additional 4 weeks. The LNCaP sublines, designated P4 and T4, were established and characterized. These sublines were co-inoculated again in castrated mice with human prostate fibroblasts for 8-12 weeks. And then second generation LNCaP sublines, P4-2 and T4-2, were established and also characterized.RESULTS:
Marked cytogenetic alterations were observed in P4-2, P4, T4-2 and T4 LNCaP sublines in comparison to parental LNCaP. Although LNCaP cells injected orthotopically did not form tumors in castrated hosts, LNCaP sublines formed PSA-producing tumors and had metastatic potentials to lymph node, lung, liver and bone. These P and T sublines had androgen-independent growth characteristics and metastatic potential.CONCLUSIONS:
Cell-cell interactions between prostatic epithelium and their surrounding fibroblasts could contribute to androgen-independent characteristics and enhanced metastatic potential of localized prostate cancer in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Prostate
/
Prostatic Neoplasms
/
Cytogenetics
/
Epithelium
/
Fibroblasts
/
Liver
/
Lung
/
Lymph Nodes
/
Mice, Nude
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS