Immunoradiometric assay of human proinsulin and partially processed proinsulin with use of monoclonal antibody and streptavidin-biotin labeling
Journal of Korean Medical Science
;
: 171-177, 1989.
Article
in English
| WPRIM
| ID: wpr-189016
ABSTRACT
The sensitive and specific immunoradiometric assay is described for human proinsulin and its intermediate peptides (65-66 split and 32-33 split proinsulin). We developed a monoclonal antibody-based two-site immunoradiometric assay with use of streptavidin-biotin labeling. The detection limits of the assays lie in the range of 0.5-2.0 pM. In the proinsulin assay proinsulin cross-reacted 66% with 65-66 split proinsulin but not with insulin or 32-33 split proinsulin. In the assay of 65-66 split proinsulin it does not cross-react with insulin, proinsulin or 32-33 split proinsulin. In the 32-33 split proinsulin assay it cross-reacted 84% with proinsulin and 60% with 65-66 split proinsulin. The precision (C.V.) of the assays was less than 15% over the various concentration. The mean concentrations of insulin, proinsulin, 65-66 split proinsulin and 32-33 proinsulin in eight young male subjects in the fasting state were (pM +/- S.E.M.) 20 +/- 3.6, 2.3 +/- 0.3, undetectable (less than 1.0) and 2.1 +/- 0.7 and at the maximum reached during an oral glucose tolerance test, 150 +/- 26, 9.9 +/- 1.4, 3.8 +/- 0.6 and 19.7 +/- 6.0 respectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proinsulin
/
Bacterial Proteins
/
Biotin
/
Immunoradiometric Assay
/
Reproducibility of Results
/
Streptavidin
/
Cross Reactions
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Journal of Korean Medical Science
Year:
1989
Type:
Article
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