The Effects of New Recombinant Protein of Fibronectin and beta ig-h3, T-CAM(Tetra Cell Adhesion Molecule) on Wound Healing

ABSTRACT

T-CAM(Tetra cell adhesion molecule) is new recombinant mixture of fibronectin and beta ig-h3. Fibronectin and beta ig-h3 are extracellular matrix protein involved in each phase of wound healing and sum of these materials may play synergistic effect on the wound healing. In order to evaluate wound healing effect of T-CAM on open wound in rabbit, we made four round full thickness skin defects, 3 cm in diameter, bilaterally on the dorsolateral aspect of New Zealand white rabbit's trunk. We divided the wound into four groups, according to the content of topocally applied onitments Group A treated with ointment base only; Group B with ointment containing fibronectin microgram/ml; Group C with ointment containing beta ig-h3 300 microgram/ml; Group D with ointment containing T-CAM 300 microgram/ml. These ointments were applied daily on the wound. We compared each group with gross findings by means of percentage of wound contraction, percentage of wound epithelization and percentage of total wound healed area with surface tracing the remained wound area on the 0, 6,th 12th, 18th day after wound formation and wound biopsy were performed on the 3rd, 6th, 10th, 14th, and 21st day after wound formation.The T-CAM group shows statistically significant (p < 0.05 ANOVA test and Scheffe's test) in wound contraction and totalwound healed area than other groups on the 6th day after wound formation, and equally significant on the 12th and 18th day after wound formation except in group C. In histological examination, T-CAM group shows less inflammatory cell infiltration, faster angiogenesis and marked fibroblast proliferation than other groups in early inflammatory period, and more matured, thickened reepithelization and regularly aligned collagen formation on the 14th and 21st day. This study suggest recombinant T-CAM shows synergistic effect on wound healing, and is expected as new potent material for treatment of wound healing.