Relationship between neuronal nitric oxide synthase and NADPH-diaphorase in the dorsal root ganglia during neuropathic pain / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 342-349, 2009.
Article
in English
| WPRIM
| ID: wpr-189222
ABSTRACT
BACKGROUND:
Changes in nitric oxide (NO) production in the dorsal root ganglia (DRG) may contribute to allodynia after nerve injury. It is known that the histochemistry of NADPH-diaphorase (NADPH-d) is known to be not always coincident with NOS. This study was conducted to investigate the relationship between nNOS and NADPH-d expression in the DRG in a spinal nerve injury model of neuropathic pain, and to elucidate role that NO plays in neuropathic pain.METHODS:
nNOS immunohistochemistry and/or NADHP-d histochemistry were conducted in the DRG of a spinal nerve transection model of neuropathic pain, and the pain behavior was then measured by a von Frey filament test of the hindpaws of wild type and nNOS knock-out mice.RESULTS:
nNOS immunoreactive neurons and NADPH-d stained neurons were not always identical. Additionally NADPH-d increased, but nNOS did not increase significantly in the DRG after spinal nerve transection. Neuropathic pain behavior increased in the hindpaw of nNOS(-/-) mice after spinal nerve transection, but was lower than that of wild type mice after spinal nerve transection.CONCLUSIONS:
nNOS immunoreactive neurons and NADPH-d stained neurons were not always identical in the DRG, and a novel NADPH-d positive source may be involved in neuropathic pain after spinal nerve transection. Changes in nNOS expression in the DRG were not the primary cause of neuropathic pain behavior in a spinal nerve transection model of neuropathic pain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spinal Nerve Roots
/
Spinal Nerves
/
Immunohistochemistry
/
Diagnosis-Related Groups
/
Mice, Knockout
/
Nitric Oxide Synthase
/
Nitric Oxide Synthase Type I
/
Ganglia, Spinal
/
Hyperalgesia
/
Neuralgia
Limits:
Animals
Language:
English
Journal:
Korean Journal of Anesthesiology
Year:
2009
Type:
Article
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