A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
The Korean Journal of Parasitology
;
: 503-510, 2013.
Article
in English
| WPRIM
| ID: wpr-189491
ABSTRACT
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Toxoplasma
/
Uganda
/
Recombinant Fusion Proteins
/
Serologic Tests
/
Antibodies, Protozoan
/
Molecular Sequence Data
/
Protozoan Proteins
/
Toxoplasmosis
/
Reproducibility of Results
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Country/Region as subject:
Africa
/
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2013
Type:
Article
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