Diagnostic Utility of a Clonality Test for Lymphoproliferative Diseases in Koreans Using the BIOMED-2 PCR Assay
Korean Journal of Pathology
;
: 458-465, 2013.
Article
in English
| WPRIM
| ID: wpr-189504
ABSTRACT
BACKGROUND:
A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain reaction (PCR) assay has been established as a standard method for assessing the clonality of LPDs. We tested clonality in LPDs in Koreans using the BIOMED-2 multiplex PCR and compared the results with those obtained in European, Taiwanese, and Thai participants. We also evaluated the usefulness of the test as an ancillary method for diagnosing LPDs.METHODS:
Two hundred and nineteen specimens embedded in paraffin, including 78 B cell lymphomas, 80 T cell lymphomas and 61 cases of reactive lymphadenitis, were used for the clonality test.RESULTS:
Mature B cell malignancies showed 95.7% clonality for IG, 2.9% co-existing clonality, and 4.3% polyclonality. Mature T cell malignancies exhibited 83.8% clonality for TCR, 8.1% co-existing clonality, and 16.2% polyclonality. Reactive lymphadenitis showed 93.4% polyclonality for IG and TCR. The majority of our results were similar to those obtained in Europeans. However, the clonality for IGK of B cell malignancies and TCRG of T cell malignancies was lower in Koreans than Europeans.CONCLUSIONS:
The BIOMED-2 multiplex PCR assay was a useful adjunctive method for diagnosing LPDs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraffin
/
Immunoglobulins
/
Receptors, Antigen, T-Cell
/
Gene Rearrangement
/
Polymerase Chain Reaction
/
Lymphoma, B-Cell
/
Lymphoma, T-Cell
/
Asian People
/
Multiplex Polymerase Chain Reaction
/
Lymphadenitis
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Korean Journal of Pathology
Year:
2013
Type:
Article
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