Validation of a Rapid, Robust, Inexpensive Screening Method for Detecting the HLA-B*58:01 Allele in the Prevention of Allopurinol-Induced Severe Cutaneous Adverse Reactions
Allergy, Asthma & Immunology Research
;
: 79-84, 2017.
Article
in English
| WPRIM
| ID: wpr-189581
ABSTRACT
The HLA B*5801 allele has been worldwide reported as a pharmacogenetic susceptibility to allopurinol-induced severe cutaneous adverse reactions (SCARs). To prevent these life-threatening conditions, the American College of Rheumatology hingly recommended that the HLA-B*5801 be screened prior to the initiation of allopurinol therapy. Therefore, we developed a rapid, robust, inexpensive screening method using SYBR® Green real time PCR to detect the HLA-B*5801 allele. A total of 119 samples were tested. The assay has a sensitivity of 100% (95% CI 69.15%-100%), a specificity of 100% (95% CI 96.67%-100%), a positive predictive value of 100% (95% CI 69.15%-100%) and a negative predictive value of 100% (95% CI 96.67%-100%). HLA-B*5801 genotyping results showed 100% agreement with those obtained from Luminex SSO/SBT/SSP. The lowest limit of detection of this method is 0.8 ng/µL of DNA. The unit cost of the test is only $3.8 USD. This novel screening test using SYBR® real time PCR would be appropriate to identify individuals with the HLA-B*5801 allele for the prevention of allopurinol-induced SCARs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rheumatology
/
DNA
/
HLA-B Antigens
/
Allopurinol
/
Mass Screening
/
Sensitivity and Specificity
/
Cicatrix
/
Stevens-Johnson Syndrome
/
Alleles
/
Limit of Detection
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2017
Type:
Article
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