Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability
Journal of the Korean Gastric Cancer Association
;
: 260-265, 2005.
Article
in Korean
| WPRIM
| ID: wpr-189860
ABSTRACT
PURPOSE:
The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers. MATERIALS ANDMETHODS:
The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene.RESULTS:
We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases.CONCLUSION:
These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Kinases
/
Stomach Neoplasms
/
DNA Damage
/
Cell Cycle
/
Frameshift Mutation
/
Microsatellite Repeats
/
Microdissection
/
Microsatellite Instability
/
Cell Cycle Checkpoints
Language:
Korean
Journal:
Journal of the Korean Gastric Cancer Association
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS