Ultrastructural Changes of Cleft Lip Muscle Following Primary Cheiloplasty

ABSTRACT

The cleft lip is the most common congenital craniofacial anomaly, the anatomical difference in operated patient and normal person by several authors. Authors deserved the ultrastructural changes in secondary cleft lip patients treated with primary cheiloplasty. We obtained eighteen muscle specimens from the upper lip of 18 patients during the time of secondary lip revision. It was stained with Hematoxylin Eosin, Epon-toluidine blue and histochemical method, and examined by light microscope. Also it was examined by electron microscope with various stain and several steps. We found the extensive collagenous fibrosis with various atrophy of muscles by light microscope, and the myofibroblast with rER, mitochondria, and abundant intermediate filaments by electron microscope in 14 cases. We also found muscular atrophy characterized with variable size of muscle fibers by light microscope, and marked variation of muscle fiber size with sarcoplasmic vacuoles, myocytolysis and internal migration of nuclei by electron microscope in 4 cases. In conclusion, in the most cases the major part of the tissues were surrounded by progressive fibrosis, the mild muscular atrophy was seen at some cases.