Emergence of CTX-M-12 and A Novel CTX-M Type Extended-Spectrum beta-Lactamaseproducing Klebsiella pneumoniae / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 21-26, 2006.
Article
in Korean
| WPRIM
| ID: wpr-190585
ABSTRACT
BACKGROUND:
The aims of this study were to survey the nation-wide susceptibilities of Klebsiella pneumoniae isolates against ceftazidime and cefotaxime and to determine the prevalence of class A extended-spectrum beta-lactamases (ESBLs).METHODS:
During the period of February to July 2004, K. pneumoniae isolates intermediate or resistant to ceftazidime and/or cefotaxime were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were determined by the disk diffusion and the agar dilution methods and ESBL-production was by double-disk synergy test. Ceftazidime or cefotaxime-resistance determinants of the ESBLproducers were transfered to Escherichia coli J53 by transconjugation. Searches for class A ESBL genes were performed by PCR amplication.RESULTS:
Among 212 clinical K. pneumoniae isolates, 172 (81%) isolates showed positive results in double-disk synergy test; the most prevalent ESBL was SHV-12 (n=104). Genes encoding ESBLs including SHV-2 (n=6), SHV-2a (n=17), CTX-M-3 (n=18), CTX-M-9 (n=6), CTX-M-12 (n=1), CTX-M- 14 (n=27), CTX-M-15 (n=3), and a novel CTX-M-type beta-lactamases were also detected.CONCLUSIONS:
It is concluded that diversity of ESBLs in K. pneumoniae isolates are increasing in Korea. CTX-M-12 has never been reported in Asia, and a novel CTX-M-type ESBL has emerged.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Asia
/
Beta-Lactamases
/
Cefotaxime
/
Ceftazidime
/
Polymerase Chain Reaction
/
Prevalence
/
Agar
/
Diffusion
/
Escherichia coli
Type of study:
Prevalence study
Country/Region as subject:
Asia
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2006
Type:
Article
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