Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy against Mycobacterium tuberculosis Infection in a Mouse Model / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 125-131, 2004.
Article
in English
| WPRIM
| ID: wpr-191079
ABSTRACT
Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over-expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN-gamma production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Tuberculosis
/
BCG Vaccine
/
Electrophoresis, Polyacrylamide Gel
/
Mycobacterium
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Limits:
Animals
/
Humans
Language:
English
Journal:
Tuberculosis and Respiratory Diseases
Year:
2004
Type:
Article
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