X-linked Gene Expression Profiles by RNAi-Mediated BRCA1 Knockdown in MCF7 Cells
Genomics & Informatics
;
: 154-158, 2005.
Article
in English
| WPRIM
| ID: wpr-191504
ABSTRACT
Germ-line mutations of the BRCA1 gene confer an increased risk for breast and ovarian cancers. BRCA1 in female cells is directly related with the maintenance of the inactive X chromosome (Xi). The effect by the loss of the BRCA1 function on the X chromosome gene expression remains unclear in cancer cells. We attempted to investigate the expression pattern of the X-linked genes by performing BRCA1 knockdown via RNA interference in the MCF 7 breast cancer cell line. The transcriptional and translational levels of BRCA1 were decreased over 95% in the MCF 7 cells after BRCA1 knockdown. The expression patterns of one hundred ninety X-linked genes were profiled by the X chromosome-specific cDNA arrays. A total of seven percent of the X-linked genes (14/190) were aberrantly expressed by over 2-fold in the MCF7-BRCA1 knockdown cells, which contained two up-regulated genes (2/190, 1%) and 12 downregulated genes (12/190, 6.3%). It is interesting that 72% of the aberrantly expressed X-linked genes were located on the Xq (10/14,) region. Our data suggests that BRCA1 may not be important to maintain X chromosome inactivation in cancer because the BRCA1 knockdown did increase the expression of the only one percent of X-linked genes in the human breast cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
X Chromosome
/
Breast
/
Breast Neoplasms
/
Gene Expression
/
Cell Line
/
Germ-Line Mutation
/
Genes, BRCA1
/
Oligonucleotide Array Sequence Analysis
/
RNA Interference
Limits:
Female
/
Humans
Language:
English
Journal:
Genomics & Informatics
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS