Evaluation of alpha-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia
The Korean Journal of Parasitology
;
: 287-291, 2009.
Article
in English
| WPRIM
| ID: wpr-191532
ABSTRACT
The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tubulin
/
Polymorphism, Restriction Fragment Length
/
Molecular Sequence Data
/
Base Sequence
/
Molecular Probes
/
Protozoan Proteins
/
Polymerase Chain Reaction
/
Sequence Alignment
/
Giardiasis
/
Giardia lamblia
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2009
Type:
Article
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