The Experience of HLA-B27 Test Using Flowcytometry / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
; : 209-213, 2002.
Article
in Ko
| WPRIM
| ID: wpr-191774
Responsible library:
WPRO
ABSTRACT
BACKGROUND: HLA-B27 is associated with an increased incidence of specific spondyloarthropathies(SpA), most notably ankylosing spondylitis(AS). I evaluated the cases referred for HLA-B27 antigen using flowcytometry (FCM) to find the clinical characteristics of the patients and the diagnostic utilities of median fluorescence intensity (MFI) in HLA-B27 program. METHODS: I evaluated 443 subjects of HLA-B27 cases using FACScan flowcytometry, consisted with software for automated calibration and analysis, calibration beads, and the anti-HLA- B27 fluorescein isothiocyanate (FITC)/anti-CD3 phycoerythrin (PE) monoclonal antibodies (all from Becton Dickinson, San Jose, CA). RESULTS: Of the total 443 cases, the positive rate in male cases was 44% (132/300) and it was higher than that of female cases (22.4%, 32/143). The gating procedure was failed in one sample of 443 (0.23%). The positive rates in each diagnostic criteria were as follows; AS 61.6%, gout 20.0%, herniated intervertebral disc 20%, lower back pain 25.6%, polyarthritis 16.0%, psoriatic arthritis 20.0%, rheumatoid arthritis 28.3%, reactive arthritis 26.9%, SpA, undifferentiated 31.8% and uveitis/iritis 23.8%. In AS group, 89 cases (95.7%) showed MFI values higher than 150. CONCLUSION: About 62% of AS group showed HLA-B27 positivity using FCM and the positive rates of other diseases group in SpA categories were around 20-30%. If we considered MFI value 150 as differential value, about 95% of HLA-B27 positive AS cases might not need further confirmatory study to differentiate HLA-B7.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phycoerythrin
/
Arthritis
/
Arthritis, Rheumatoid
/
Spondylitis, Ankylosing
/
Calibration
/
Arthritis, Psoriatic
/
HLA-B7 Antigen
/
HLA-B27 Antigen
/
Incidence
/
Arthritis, Reactive
Type of study:
Incidence_studies
/
Prognostic_studies
Limits:
Female
/
Humans
/
Male
Language:
Ko
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2002
Type:
Article