Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil
Journal of Veterinary Science
;
: 241-248, 2014.
Article
in English
| WPRIM
| ID: wpr-191844
ABSTRACT
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlandia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlandia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlandia and Sao Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Outer Membrane Proteins
/
Bacterial Proteins
/
Brazil
/
Molecular Sequence Data
/
RNA, Ribosomal, 16S
/
Base Sequence
/
Polymerase Chain Reaction
/
Sequence Alignment
/
Ehrlichiosis
/
Fluorescent Antibody Technique, Indirect
Type of study:
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Journal of Veterinary Science
Year:
2014
Type:
Article
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