GFP-tagged E. coli shows bacterial distribution in mouse organs: pathogen tracking using fluorescence signal
Clinical and Experimental Vaccine Research
;
: 83-87, 2012.
Article
in English
| WPRIM
| ID: wpr-192288
ABSTRACT
PURPOSE:
In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the in vivo information within consistent background with identical organism. MATERIALS ANDMETHODS:
Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating Escherichia coli MC1061 strain which expresses GFP (E. coli-GFP) in BALB/C mice after injection.RESULTS:
Local distribution of disseminated E. coli-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal.CONCLUSION:
This in vivo imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sprains and Strains
/
Bacterial Infections
/
Track and Field
/
Escherichia coli
/
Molecular Imaging
/
Fluorescence
/
Intestines
Limits:
Animals
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2012
Type:
Article
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