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B cell-associated immune profiles in patients with end-stage renal disease (ESRD)
Article in English | WPRIM | ID: wpr-192556
Responsible library: WPRO
ABSTRACT
Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused on T cell immunity. We investigated B cell subpopulations in ESRD patients and the effect of hemodialysis (HD) on B cell-associated immune profiles in these patients. Forty-four ESRD [maintenance HD patients (n = 27) and pre-dialysis patients (n = 17)] and 27 healthy volunteers were included in this study. We determined the percentage of B cell subtypes, such as mature and immature B cells, memory B cells, and interleukin (IL)-10+ cells, as well as B cell-producing cytokines (IL-10, IL-4 and IL-21) by florescent activated cell sorting (FACS). B cell-associated gene expression was examined using real-time PCR and B cell producing cytokines (IL-10, IL-4 and IL-21) were determined using an enzyme-linked immunosorbent assay (ELISA). The percentage of total B cells and mature B cells did not differ significantly among the three groups. The percentages of memory B cells were significantly higher in the pre-dialysis group than in the HD group (P 0.05) between the two subgroups within the ESRD group, but the serum IL-10 concentration was significantly lower in the pre-dialysis group (P < 0.01). The results of this study demonstrate significantly altered B cell-associated immunity. Specifically, an imbalance of immature and memory B cells in ESRD patients was observed, with this finding predominating in pre-dialysis patients.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Female / Humans / Male / Leukocytes, Mononuclear / B-Lymphocytes / Cytokines / Immunophenotyping / B-Lymphocyte Subsets / Proto-Oncogene Proteins / Interleukin-10 Language: English Journal: Experimental & Molecular Medicine Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Female / Humans / Male / Leukocytes, Mononuclear / B-Lymphocytes / Cytokines / Immunophenotyping / B-Lymphocyte Subsets / Proto-Oncogene Proteins / Interleukin-10 Language: English Journal: Experimental & Molecular Medicine Year: 2012 Type: Article