Effects of 630 nm Light-Emitting Diode Irradiation on Caveolin-1 and Procollagen I and III Expression in Human Dermal Fibroblasts / 대한피부과학회지
Korean Journal of Dermatology
;
: 161-168, 2014.
Article
in Korean
| WPRIM
| ID: wpr-192884
ABSTRACT
BACKGROUND:
Recent studies indicate that light-emitting diodes (LED) may represent a novel and effective anti-aging light source for the skin. Among many candidate molecules known to control collagens, caveolin-1 (Cav-1) is known to play an inhibitory role in cutaneous collagen metabolism.OBJECTIVE:
This study aimed to evaluate the effects of LED irradiation on the expression levels of Cav-1 and procollagens (proCOLs) in human dermal fibroblasts (HDFs).METHODS:
Cultured HDFs were irradiated with 630 nm LED at different doses, and the mRNA and protein expression levels of Cav-1 and proCOLs I/III were analyzed.RESULTS:
In LED-irradiated HDFs, mRNA and protein levels of Cav-1 were found to be down-regulated, whereas those of proCOLs I/III were up-regulated in a dose-dependent manner. A negative correlation between Cav-1 and proCOLs was verified in Cav-1 siRNA transfected HDFs. LED was moreover found to result in up-regulation of transforming growth factor (TGF)-beta1 and its receptors (TbetaRI, TbetaRII), SMAD1, and SMAD2 mRNA levels, indicating that LED may activate the TGF-1/TbetaR/SMAD pathway in HDFs.CONCLUSION:
The anti-aging effects of 630 nm LED on human skin are likely mediated by up-regulation of proCOLs I/III and inhibition of Cav-1 expression levels in HDFs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
RNA, Messenger
/
Transforming Growth Factors
/
Up-Regulation
/
Collagen
/
Procollagen
/
RNA, Small Interfering
/
Caveolin 1
/
Fibroblasts
/
Metabolism
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2014
Type:
Article
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