Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immmunogens III. Characterization of immunodominant peptides
The Korean Journal of Parasitology
;
: 111-116, 1994.
Article
in English
| WPRIM
| ID: wpr-193164
ABSTRACT
Immunoblot analysis utilizing bovine sera from naturally or experimentally infected with Theileria sergenti were used to determine the immunodominant polypeptides of T. sergenti (Korea isolate).The previously recognized major bands, 18 kDa, 29 kDa, 34 kDa, and 45 kDa, were excised after electrophoresis and trasferred to PVDF membrane. The individual bands were sequenced. The 34 kDa polypeptide which was the most antigenic and immunogenic peptide was observed in the Western blot. However, Chou-Fasman prediction sites (antiginic site) for antigen determinants of the 45 kDa,34 kDa, 29 kDa and 18 kDa polypeptide were 6, 4, 2 and 0, respectively. However, the 45 kDa polypeptide showed no reaction with anti-T. sergenti hyperimmune serum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Amino Acid Sequence
/
Amino Acids
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
1994
Type:
Article
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