Compiling Multicopy Single-Stranded DNA Sequences from Bacterial Genome Sequences
Genomics & Informatics
;
: 29-33, 2016.
Article
in English
| WPRIM
| ID: wpr-193407
ABSTRACT
A retron is a bacterial retroelement that encodes an RNA gene and a reverse transcriptase (RT). The former, once transcribed, works as a template primer for reverse transcription by the latter. The resulting DNA is covalently linked to the upstream part of the RNA; this chimera is called multicopy single-stranded DNA (msDNA), which is extrachromosomal DNA found in many bacterial species. Based on the conserved features in the eight known msDNA sequences, we developed a detection method and applied it to scan National Center for Biotechnology Information (NCBI) RefSeq bacterial genome sequences. Among 16,844 bacterial sequences possessing a retron-type RT domain, we identified 48 unique types of msDNA. Currently, the biological role of msDNA is not well understood. Our work will be a useful tool in studying the distribution, evolution, and physiological role of msDNA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biotechnology
/
DNA
/
DNA, Single-Stranded
/
RNA
/
Chimera
/
RNA-Directed DNA Polymerase
/
Genome, Bacterial
/
Retroelements
/
Reverse Transcription
Language:
English
Journal:
Genomics & Informatics
Year:
2016
Type:
Article
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