The difference of interieukin - 4 and interferon - r production of Der p I stimulated T cells and effects of immunomodulator in house dust mite sensitive atopic and non - atopic individuals / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
;
: 548-556, 1999.
Article
in Korean
| WPRIM
| ID: wpr-193529
ABSTRACT
BACKGROUND:
Allergic diseases are characterized by immediate - and late - phase reactions to various allergens by the selective activation of a subset of CD4 + T cells. In response to allergen, T cells isolated from atopic donors are biased to low levels of IFN - y and high levels of IL - 4, and vice versa by T cells from non - atopic donor.Objective:
The aim of this study was to evaluate the patterns of IL - 4 and IFN - y production after Der p I stimulation and the effect on the cytokine production from T cells by budesonide, disodium cromoglycate and cyclosporin A in atopic and non - atopic individuals. MATERIAL ANDMETHOD:
Seven Der p I specific atopic and 7 non - atopic individuals were selected. We decided the 50% inhibiting concentration of each immunomodulator by lymphocyte proliferation assay, and measured their effects on the cytokine production in vitro by intracellular IL - 4, IFN - y staining and flow cytometry. Results andCONCLUSION:
There was significant difference on stimulation index ( SI ) of production of IFN - y as well as IL-4 after Der p I stimulation between atopic and non-atopic individuals ( IL - 4 ; 1.57 +/- 0.7 0.98 +/- 0.2, p = 0.026, IFN - r 1.45 +/- 0.5 0.95 +/- 0.2, p = 0.048 ). The synthesis of IL - 4, and IFN - r were significantly inhibited after the stimulation of every immunomodulators in atopic individuals, DSCG couldnt inhibit IL - 4 and IFN - r in nonatopic individuals. There was no significant difference in the inhibiting effect of these immunomodulators in both of them.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
Allergens
/
Lymphocytes
/
T-Lymphocytes
/
Bias
/
Interferons
/
Interleukin-4
/
Cyclosporine
/
Cromolyn Sodium
/
Budesonide
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Asthma, Allergy and Clinical Immunology
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS