Mycobacterial Infection after Intravesical Bacillus Calmette-Guerin Treatment for Bladder Cancer: A Case Report / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 197-200, 2011.
Article
in English
| WPRIM
| ID: wpr-193617
ABSTRACT
Bacillus Calmette-Guerin (BCG) has been traditionally used as a vaccine against tuberculosis. Further, intravesical administration of BCG has been shown to be effective in treating bladder cancer. Although BCG contains a live attenuated strain of Mycobacterium bovis, complications such as M. bovis BCG infection caused by BCG administration are extremely rare. Here, we report a case of BCG infection occurring after intravesical BCG therapy. A 67-yr-old man presented with azotemia and weight loss. He had been diagnosed with bladder cancer 4 yr back, and had undergone transurethral resection of the bladder tumor and intravesical BCG (Tice strain) therapy at that time. An acid-fast bacterial strain was isolated from his urine sample. We did not detect Mycobacterium tuberculosis protein 64 (MPT-64) antigen in the isolates obtained from his sample, and multiplex PCR and PCR-reverse blot hybridization assay indicated that the isolate was a member of the M. tuberculosis complex, but was not M. tuberculosis. Finally, sequence analysis of 16S ribosomal RNA and DNA gyrase, subunit B (gyrB) suggested that the organism was M. bovis or M. bovis BCG. Although we could not confirm that M. bovis BCG was the causative agent, the results of the 3 molecular methods and the MPT-64 antigen assay suggest this finding. This is an important finding, especially because M. bovis BCG cannot be identified using common commercial molecular genetics tools.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urinary Bladder Neoplasms
/
Administration, Intravesical
/
RNA, Ribosomal, 16S
/
BCG Vaccine
/
Polymerase Chain Reaction
/
DNA Gyrase
/
Mycobacterium bovis
/
Mycobacterium Infections
Type of study:
Prognostic study
Limits:
Aged
/
Humans
/
Male
Language:
English
Journal:
The Korean Journal of Laboratory Medicine
Year:
2011
Type:
Article
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