Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination
Journal of Veterinary Science
;
: 421-425, 2016.
Article
in English
| WPRIM
| ID: wpr-193783
ABSTRACT
Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that of the RT-LAMP without a UNG treatment. To the best of our knowledge, this is the first successful application of deoxyuridine triphosphate/UNG strategy in RT-LAMP for AIV detection, and the assay can be applied for the rapid, and reliable diagnosis of AIVs, even in contaminated samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Deoxyuridine
/
Reverse Transcription
/
Diagnosis
/
Uracil-DNA Glycosidase
/
Limit of Detection
/
Influenza in Birds
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2016
Type:
Article
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