The Effect of Heparin Binding Epidermal Growth Factor on the Hypertrophy / Hyperplasia of Human Bladder Smooth Muscle
Journal of the Korean Continence Society
;
: 169-177, 2008.
Article
in Korean
| WPRIM
| ID: wpr-193994
ABSTRACT
PUROPOSE The bladder outlet obstruction (BOO) causes a bladder hypertrophy and/or hyperplasia with time. Heparin binding epidermal growth factor like growth factor (HB-EGF) is a potent mitogen for bladder smooth muscle cell (BMSC), causing significant cellular hypertrophy. Few studies have evaluated the effect of HB-EGF on bladder hypertrophy or hyperplasia. We studied to evaluated the effect of HB-EGF on the hypertrophy or hyperplasia of human BSMC and fibroblast. MATERIAL AND METHODS:
To evaluate the change of expression of HB-EGF mRNA of human BMSC under a constant stimuli of 40cm-H20 pressure during 0, 1, 3 and 5 hours, RT-PCR was perfromed. The human BMSC and bladder fibroblasts with the addition of the recombinant HB-EGF 25ng/ml were performed 3H-thymidine and 3H-leucine incorporation assay for DNA and protein production, respectively.RESULTS:
The expression of HB-EGF mRNA in BMSC under a constant stimuli of 40cm-H20 pressure was up-regulated with time (p<0.05). The optimal concentration of HB-EGF was 25ng/ml. The 3H-Lecine and 3H-thymidine activity in both human BSMC and fibroblast were significantly increased in addition of 25ng/ml of HB-EGF (p<0.05 and p<0.05, respectively).CONCLUSIONS:
Constant hydrostatic pressure induces the expression of HB-EGF in human BSMC. Subsequently HB-EGF induces hypertrophy and hyperplasia of human BSMC and fibroblast in vitro. Our data support that HB-EGF is one of relevant BOO-induced growth factors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urinary Bladder
/
Urinary Bladder Neck Obstruction
/
DNA
/
RNA, Messenger
/
Heparin
/
Myocytes, Smooth Muscle
/
Intercellular Signaling Peptides and Proteins
/
Epidermal Growth Factor
/
Fibroblasts
/
Hydrostatic Pressure
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Continence Society
Year:
2008
Type:
Article
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