Chlorogenic Acid Decreases Retinal Vascular Hyperpermeability in Diabetic Rat Model
Journal of Korean Medical Science
;
: 608-613, 2013.
Article
in English
| WPRIM
| ID: wpr-194140
ABSTRACT
To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retina
/
Blood-Retinal Barrier
/
Down-Regulation
/
Chlorogenic Acid
/
Dextrans
/
Fluorescein-5-isothiocyanate
/
Rats, Sprague-Dawley
/
Vascular Endothelial Growth Factor A
/
Diabetes Mellitus, Experimental
/
Diabetic Retinopathy
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS