Alpha-Type 1 Polarized Dendritic Cells Loaded with Apoptotic Allogeneic Breast Cancer Cells Can Induce Potent Cytotoxic T Lymphocytes against Breast Cancer / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 56-66, 2011.
Article
in English
| WPRIM
| ID: wpr-194256
ABSTRACT
PURPOSE:
Various tumor antigens can be loaded onto dendritic cells (DCs) to induce a potent cytotoxic T lymphocyte (CTL) response in DC-based immunotherapy against breast cancer. However, in the clinical setting, obtaining a sufficient number of autologous tumor cells as a source of tumor antigens is a laborious process. We therefore investigated the feasibility of immunotherapy using breast-cancer-specific CTLs generated in vitro by use of alpha-type 1 polarized DCs (alpha DC1s) loaded with ultraviolet B-irradiated cells of the breast cancer cell line MCF-7. MATERIALS ANDMETHODS:
alphaDC1s were induced by loading allogeneic tumor antigen generated from the MCF-7 UVB-irradiated breast cancer cell line. Antigen-pulsed alphaDC1s were evaluated by morphological and functional assays, and the breast-cancer-specific CTL response was analyzed by cytotoxic assay.RESULTS:
The alphaDC1s significantly increased the expression of several molecules related to DC maturation without differences according to whether the alphaDC1s were loaded with tumor antigens. The alphaDC1s showed a high production of interleukin-12 both during maturation and after subsequent stimulation with CD40L, which was not significantly affected by loading with tumor antigens. Breast-cancer-specific CTLs against autologous breast cancer cells were successfully induced by alphaDC1s loaded with apoptotic MCF-7 cells.CONCLUSION:
Autologous DCs loaded with an allogeneic breast cancer cell line can generate potent breast-cancer-specific CTL responses. This may be a practical method for cellular immunotherapy in patients with breast cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Dendritic Cells
/
Breast
/
Breast Neoplasms
/
Lymphocytes
/
T-Lymphocytes, Cytotoxic
/
Cell Line
/
Interleukin-12
/
CD40 Ligand
/
Immunotherapy
/
Antigens, Neoplasm
Limits:
Humans
Language:
English
Journal:
Cancer Research and Treatment
Year:
2011
Type:
Article
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