Cloning of Danio rerio toll-like receptor 1 / 나학회지
Korean Leprosy Bulletin
;
: 3-14, 2005.
Article
in Korean
| WPRIM
| ID: wpr-194545
ABSTRACT
Toll-like receptors (TLRs) play a central role of innate immunity as well as of enhancing the adaptive immunity. TLR1 interacts with TLR2 to recognize the mycobacterial 19-kD lipoprotein. Danio rerio genomic sequences predicted TLR family genes. Danio rerio TLR1 gene was cloned with RACE-PCR and RT-PCR methods to get more sights of immunity against mycobacterial infection such as leprosy. Cloned TLR1 showed insertion of 22 amino acid residues comparing to NCBI- predicted TLR1, and an open reading frame composing of deduced 795 amino acids. Toll-interlukin1 receptor domain and leucine-rich repeats were in the cloned TLR1 as were noted in NCBI-predicted TLR1. Cloned zebrafish TLR1 had high similarity to human TLR1 with 35% amino acid identities and 57% amino acid positivities across the entire sequence (E=1e-130). The cloned sequence might help to study the innate and adaptive immunity of mycobacterial infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Zebrafish
/
Open Reading Frames
/
Clone Cells
/
Cloning, Organism
/
Toll-Like Receptors
/
Toll-Like Receptor 1
/
Adaptive Immunity
/
Amino Acids
/
Immunity, Innate
/
Leprosy
Limits:
Humans
Language:
Korean
Journal:
Korean Leprosy Bulletin
Year:
2005
Type:
Article
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