Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells / 소아과
Korean Journal of Pediatrics
;
: 881-885, 2005.
Article
in Korean
| WPRIM
| ID: wpr-195108
ABSTRACT
PURPOSE:
Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils.METHODS:
Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1.RESULTS:
Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium.CONCLUSION:
We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma, Exercise-Induced
/
Cell Line
/
Bronchoconstriction
/
Superoxides
/
Intercellular Adhesion Molecule-1
/
Dehydration
/
Eosinophils
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Pediatrics
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS