Steroid-Induced Ocular Hypertension Model in the Mice
Journal of the Korean Ophthalmological Society
;
: 1202-1207, 2014.
Article
in Korean
| WPRIM
| ID: wpr-195450
ABSTRACT
PURPOSE:
To determine whether rat eyes develop increases in intraocular pressure (IOP) in response to a topically applied corticosteroid and to investigate the relationship between ocular hypertension and apoptosis of retinal ganglion cells.METHODS:
IOP was monitored by rebound tonometry in a group of 10 rats that received topically administered dexamethasone in both eyes (experimental) and in another group of 5 rats that received artificial tears (control) three times daily for 4 weeks after the establishment of baseline IOP values. Only eyes that increased by more than 50% compared with the basal IOP were administered once per day for 5 weeks. After 8 weeks, selective immunofluorescence stain for retinal ganglion cells and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain were conducted.RESULTS:
Among 20 experimental eyes, 11 eyes (55%) showed a greater than 50% increase in IOP compared with basal IOP. After 8 weeks, the mean IOPs for the experimental and control groups were 11.8 +/- 1.4 mm Hg and 18.5 +/- 1.0 mm Hg, respectively (p < 0.01). The counts of central retinal ganglion cells (RGCs) were 2718 +/- 240 and 2612 +/- 443, respectively (p = 0.294). The results of the TUNEL stain also showed no differences.CONCLUSIONS:
Rat eyes exhibit a steroid-induced ocular hypertensive response with no local complications. However, maintaining ocular hypertension increased by 50% for two months was not enough to detect changes in RGCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ophthalmic Solutions
/
Retinal Ganglion Cells
/
Dexamethasone
/
Ocular Hypertension
/
Fluorescent Antibody Technique
/
Apoptosis
/
In Situ Nick-End Labeling
/
DNA Nucleotidylexotransferase
/
Intraocular Pressure
/
Manometry
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2014
Type:
Article
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